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LearnTA500876
antibody from OriGene
Targeting: GRIPAP1
DKFZp434P0630, GRASP-1, GRASP1, KIAA1167, MPMGp800B12492Q3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- TA500876 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500876, RRID:AB_11126479
- Product name
- Anti-GRIPAP1 (GRASP1) mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
- Antibody type
- Monoclonal
- Description
- Anti-GRIPAP1 (GRASP1) mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GRIPAP1
- Isotype
- IgG
- Antibody clone number
- OTI1E3
- Vial size
- 100 µl
- Concentration
- 0.82 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GRIPAP1 mouse monoclonal antibody (TA500876) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GRIPAP1(RC210936).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-GRIPAP1 mouse monoclonal antibody (TA500876).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GRIPAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500876, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500876, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500876, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500876, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500876, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-GRIPAP1 antibody(TA500876),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
