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LearnCF500876
antibody from Invitrogen Antibodies
Targeting: GRIPAP1
DKFZp434P0630, GRASP-1, GRASP1, KIAA1167, MPMGp800B12492Q3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- CF500876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRIPAP1 Monoclonal Antibody (OTI1E3), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1E3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIP. Positive lysates LY402754 (100 µg) and LC402754 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRIPAP1 (RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIP. Positive lysates LY402754 (100 µg) and LC402754 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-GRIPAP1 mouse monoclonal antibody (TA500876).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-GRIPAP1 mouse monoclonal antibody (TA500876) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GRIPAP1(RC210936).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-GRIPAP1 mouse monoclonal antibody (TA500876).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-GRIPAP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500876, Dilution 1:50)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-GRIPAP1 antibody (TA500876), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-GRIPAP1 antibody (TA500876), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).
