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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4150 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4150, RRID:AB_1579026
- Product name
- PPP2CA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PPP2CA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function.
Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits.
The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic.
Gergs U, Boknik P, Buchwalow I, Fabritz L, Matus M, Justus I, Hanske G, Schmitz W, Neumann J
The Journal of biological chemistry 2004 Sep 24;279(39):40827-34
The Journal of biological chemistry 2004 Sep 24;279(39):40827-34
Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits.
Prickett TD, Brautigan DL
The Journal of biological chemistry 2004 Sep 10;279(37):38912-20
The Journal of biological chemistry 2004 Sep 10;279(37):38912-20
The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic.
Scott GK, Gu F, Crump CM, Thomas L, Wan L, Xiang Y, Thomas G
The EMBO journal 2003 Dec 1;22(23):6234-44
The EMBO journal 2003 Dec 1;22(23):6234-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PPP2CA polyclonal antibody (Cat # PAB4150) in CEM cell lysate (35 ug/lane). PPP2CA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with PPP2CA polyclonal antibody (Cat # PAB4150) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
