Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 13482-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP2CA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDEKVFTKE LDQWIEQLNE CKQLSESQVK SLCEKAKEIL TKESNVQEVR CPVTVCGDVH GQFHDLMELF RIGGKSPDTN YLFMGDYVDR GYYSVETVTL LVALKVRYRE RITILRGNHE SRQITQVYGF YDECLRKYGN ANVWKYFTDL FDYLPLTALV DGQIFCLHGG LSPSIDTLDH IRALDRLQEV PHEGPMCDLL WSDPDDRGGW GISPRGAGYT FGQDISETFN HANGLTLVSR AHQLVMEGYN WCHDRNVVTI FSAPNYCYRC GNQAAIMELD DTLKYSFLQF DPAPRRGEPH VTRRTPDYFL (1-309 aa encoded by BC031696)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 13482-1-AP (PPP2CA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PPP2CA (13482-1-AP for IP and Detection) with HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PPP2CA (IP:13482-1-AP, 4 ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400 ug.