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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-31266 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31266, RRID:AB_2284353
- Product name
- Rabbit Polyclonal PP2A alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPP2R4 Antibody [NBP1-31266] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPP2R4 Antibody [NBP1-31266] - Sample (30 ug of whole cell lysate) A: 293T B: Molt-4 10% SDS PAGE, antibody diluted at 1:1500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NBP1-31266] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PP2A alpha Antibody [NBP1-31266] - Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 ug of PP2A alpha antibody Western blot analysis was performed using PP2A alpha antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-31266] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
