Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-844 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-844, RRID:AB_2237563
- Product name
- Goat Polyclonal PP2A alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Two variants NP_001009552.1 and NP_004147.1 represent identical protein.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR
Nucleic acids research 1988 Dec 9;16(23):11366
Nucleic acids research 1988 Dec 9;16(23):11366
The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR
Nucleic acids research 1988 Dec 9;16(23):11366
Nucleic acids research 1988 Dec 9;16(23):11366
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-844] - Staining (0.1ug/ml) of Mouse (A), Rat (B) and Pig (C) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-844] - Staining (0.05ug/ml) of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - )Staining of paraffin embedded Human Thyroid. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - Staining of paraffin embedded Human Kidney. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.