AF4589

antibody from R&D Systems

Targeting: PTK2B CADTK, CAKB, FAK2, PTK, PYK2, RAFTK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4589

Provider

R&D Systems

Product name

Human PYK2/FAK2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human PYK2 in Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q14289


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human PYK2/FAK2 by Western Blot. Western blot shows lysates of human brain tissue, Daudi human Burkitt lymphoma, and A549 human lung carcinoma cells. PVDF membrane was probed with 1 µg/mL of Human PYK2/FAK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PYK2/FAK2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

PYK2/FAK2 in Human Brain. PYK2/FAK2 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human PYK2/FAK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4589) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.