M01941

antibody from Boster Biological Technology

Targeting: HTRA2 OMI, PARK13, PRSS25

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M01941

Provider

Boster Biological Technology

Product name

Anti-HTRA2/Omi Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Omi/HTRA2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IHC, WB. Reactive species: Human, Mouse, Rat Omi/HTRA2 information: Molecular Weight: 48841 MW; Subcellular Localization: Mitochondrion intermembrane space. Mitochondrion membrane ; Single-pass membrane protein . Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID; Tissue Specificity: Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EEH-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of HTRA2 expression in Jurkat cell lysate (M01941). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA2 monoclonal antibody (Catalog # M01941) overnight at 4

Additional image