Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM09369PU-S - Provider product page
- Provider
- OriGene
- Product name
- Bak (BAK1) mouse monoclonal antibody, clone AT8B4, Purified
- Antibody type
- Monoclonal
- Description
- Bak (BAK1) mouse monoclonal antibody, clone AT8B4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BAK1
- Isotype
- IgG
- Antibody clone number
- AT8B4
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Mouse lung lysates (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BAK1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human BAK1 antibody (1:500) with Texas Red (Red).
- Validation comment
- IF