Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- M01163-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-BAK/BAK1 Antibody Picoband™ (monoclonal, 4C2)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for BAK/BAK1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4C2
- Vial size
- 100μg/vial
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references Extracellular ATP contributes to the reactive oxygen species burst and exaggerated mitochondrial damage in D-galactosamine and lipopolysaccharide-induced fulminant hepatitis.
BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells.
Xu R, Yuan LS, Gan YQ, Lu N, Li YP, Zhou ZY, Hu B, Wong TS, He XH, Zha QB, Ouyang DY
International immunopharmacology 2024 Mar 30;130:111680
International immunopharmacology 2024 Mar 30;130:111680
BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells.
Tong QS, Zheng LD, Wang L, Liu J, Qian W
BMC cancer 2004 Jul 12;4:33
BMC cancer 2004 Jul 12;4:33
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of BAK1 using anti-BAK1 antibody (M01163-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human A431 whole cell lysates Lane 3: human SW620 whole cell lysates Lane 4: human T-47D whole cell lysates Lane 5: human HepG2 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAK1 antigen affinity purified monoclonal antibody (Catalog # M01163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BAK1 at approximately 23-25KD. The expected band size for BAK1 is at 23-25KD.
- Additional image