Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF816 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BAK Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BAK in Western blots. This antibody does not detect mouse BAK.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q16611- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A redox state-dictated signalling pathway deciphers the malignant cell specificity of CD40-mediated apoptosis.
Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
Dunnill CJ, Ibraheem K, Mohamed A, Southgate J, Georgopoulos NT
Oncogene 2017 May 4;36(18):2515-2528
Oncogene 2017 May 4;36(18):2515-2528
Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
Karrar A, Broomé U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
Gut 2007 Feb;56(2):243-52
Gut 2007 Feb;56(2):243-52
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN
Cancer research 2004 May 1;64(9):3022-9
Cancer research 2004 May 1;64(9):3022-9
Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN
Cancer research 2004 May 1;64(9):3022-9
Cancer research 2004 May 1;64(9):3022-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BAK by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1-4 µg/mL of Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BAK at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BAK in HEK293 Human Cell Line. BAK was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
