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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8008 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8008, RRID:AB_628017
- Product name
- Anti-RELA
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Lycopene Inhibits NF-kB-Mediated IL-8 Expression and Changes Redox and PPARγ Signalling in Cigarette Smoke-Stimulated Macrophages.
FKBP51 protects 661w cell culture from staurosporine-induced apoptosis.
NF-kB and c-Jun induce the expression of the oncogenic miR-221 and miR-222 in prostate carcinoma and glioblastoma cells.
Endotoxin-activated microglia injure brain derived endothelial cells via NF-κB, JAK-STAT and JNK stress kinase pathways.
Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding Interface of the B14 Protein, a Vaccinia Virus Inhibitor of IKK{beta}-mediated Activation of Nuclear Factor {kappa}B.
Discovery of non-ETS gene fusions in human prostate cancer using next-generation RNA sequencing.
Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling.
Breviscapine attenuates acute pancreatitis by inhibiting expression of PKCα and NF-κB in pancreas.
NFX1 plays a role in human papillomavirus type 16 E6 activation of NFkappaB activity.
Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice.
Silencing of the JNK pathway maintains progesterone receptor activity in decidualizing human endometrial stromal cells exposed to oxidative stress signals.
p53-mediated delayed NF-κB activity enhances etoposide-induced cell death in medulloblastoma.
Nuclear factor (NF)-kappaB-dependent thyroid hormone receptor beta1 expression controls dendritic cell function via Akt signaling.
Porcine endothelial cells cocultured with smooth muscle cells became procoagulant in vitro.
Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes.
Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms.
Anti-inflammatory effects of the 70 kDa heat shock protein in experimental stroke
Differential regulation of Mn-superoxide dismutase in neurons and astroglia by HIV-1 gp120: Implications for HIV-associated dementia.
Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing in osteoclast precursors expression of LFA-1 and ICAM-1.
NF-kappa B activation in human breast cancer specimens and its role in cell proliferation and apoptosis.
Simone RE, Russo M, Catalano A, Monego G, Froehlich K, Boehm V, Palozza P
PloS one 2011;6(5):e19652
PloS one 2011;6(5):e19652
FKBP51 protects 661w cell culture from staurosporine-induced apoptosis.
Daudt DR, Yorio T
Molecular vision 2011;17:1172-81
Molecular vision 2011;17:1172-81
NF-kB and c-Jun induce the expression of the oncogenic miR-221 and miR-222 in prostate carcinoma and glioblastoma cells.
Galardi S, Mercatelli N, Farace MG, Ciafrè SA
Nucleic acids research 2011 May;39(9):3892-902
Nucleic acids research 2011 May;39(9):3892-902
Endotoxin-activated microglia injure brain derived endothelial cells via NF-κB, JAK-STAT and JNK stress kinase pathways.
Kacimi R, Giffard RG, Yenari MA
Journal of inflammation (London, England) 2011 Mar 7;8:7
Journal of inflammation (London, England) 2011 Mar 7;8:7
Mapping the I{kappa}B Kinase {beta} (IKK{beta})-binding Interface of the B14 Protein, a Vaccinia Virus Inhibitor of IKK{beta}-mediated Activation of Nuclear Factor {kappa}B.
Benfield CT, Mansur DS, McCoy LE, Ferguson BJ, Bahar MW, Oldring AP, Grimes JM, Stuart DI, Graham SC, Smith GL
The Journal of biological chemistry 2011 Jun 10;286(23):20727-35
The Journal of biological chemistry 2011 Jun 10;286(23):20727-35
Discovery of non-ETS gene fusions in human prostate cancer using next-generation RNA sequencing.
Pflueger D, Terry S, Sboner A, Habegger L, Esgueva R, Lin PC, Svensson MA, Kitabayashi N, Moss BJ, MacDonald TY, Cao X, Barrette T, Tewari AK, Chee MS, Chinnaiyan AM, Rickman DS, Demichelis F, Gerstein MB, Rubin MA
Genome research 2011 Jan;21(1):56-67
Genome research 2011 Jan;21(1):56-67
Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling.
Levy D, Kuo AJ, Chang Y, Schaefer U, Kitson C, Cheung P, Espejo A, Zee BM, Liu CL, Tangsombatvisit S, Tennen RI, Kuo AY, Tanjing S, Cheung R, Chua KF, Utz PJ, Shi X, Prinjha RK, Lee K, Garcia BA, Bedford MT, Tarakhovsky A, Cheng X, Gozani O
Nature immunology 2011 Jan;12(1):29-36
Nature immunology 2011 Jan;12(1):29-36
Breviscapine attenuates acute pancreatitis by inhibiting expression of PKCα and NF-κB in pancreas.
Zhang H, Cai CZ, Zhang XQ, Li T, Jia XY, Li BL, Song L, Ma XJ
World journal of gastroenterology : WJG 2011 Apr 14;17(14):1825-30
World journal of gastroenterology : WJG 2011 Apr 14;17(14):1825-30
NFX1 plays a role in human papillomavirus type 16 E6 activation of NFkappaB activity.
Xu M, Katzenellenbogen RA, Grandori C, Galloway DA
Journal of virology 2010 Nov;84(21):11461-9
Journal of virology 2010 Nov;84(21):11461-9
Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice.
Wang F, Okamoto Y, Inoki I, Yoshioka K, Du W, Qi X, Takuwa N, Gonda K, Yamamoto Y, Ohkawa R, Nishiuchi T, Sugimoto N, Yatomi Y, Mitsumori K, Asano M, Kinoshita M, Takuwa Y
The Journal of clinical investigation 2010 Nov 1;120(11):3979-95
The Journal of clinical investigation 2010 Nov 1;120(11):3979-95
Silencing of the JNK pathway maintains progesterone receptor activity in decidualizing human endometrial stromal cells exposed to oxidative stress signals.
Leitao B, Jones MC, Fusi L, Higham J, Lee Y, Takano M, Goto T, Christian M, Lam EW, Brosens JJ
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1541-51
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 May;24(5):1541-51
p53-mediated delayed NF-κB activity enhances etoposide-induced cell death in medulloblastoma.
Meley D, Spiller DG, White MR, McDowell H, Pizer B, Sée V
Cell death & disease 2010 May 13;1(5):e41
Cell death & disease 2010 May 13;1(5):e41
Nuclear factor (NF)-kappaB-dependent thyroid hormone receptor beta1 expression controls dendritic cell function via Akt signaling.
Mascanfroni ID, Montesinos Mdel M, Alamino VA, Susperreguy S, Nicola JP, Ilarregui JM, Masini-Repiso AM, Rabinovich GA, Pellizas CG
The Journal of biological chemistry 2010 Mar 26;285(13):9569-82
The Journal of biological chemistry 2010 Mar 26;285(13):9569-82
Porcine endothelial cells cocultured with smooth muscle cells became procoagulant in vitro.
Pang Z, Niklason LE, Truskey GA
Tissue engineering. Part A 2010 Jun;16(6):1835-44
Tissue engineering. Part A 2010 Jun;16(6):1835-44
Trypanosoma cruzi induces the reactive oxygen species-PARP-1-RelA pathway for up-regulation of cytokine expression in cardiomyocytes.
Ba X, Gupta S, Davidson M, Garg NJ
The Journal of biological chemistry 2010 Apr 9;285(15):11596-606
The Journal of biological chemistry 2010 Apr 9;285(15):11596-606
Binding sites for ETS family of transcription factors dominate the promoter regions of differentially expressed genes in abdominal aortic aneurysms.
Nischan J, Gatalica Z, Curtis M, Lenk GM, Tromp G, Kuivaniemi H
Circulation. Cardiovascular genetics 2009 Dec;2(6):565-72
Circulation. Cardiovascular genetics 2009 Dec;2(6):565-72
Anti-inflammatory effects of the 70 kDa heat shock protein in experimental stroke
Zhen Zheng, Jong Youl Kim, Hualong Ma, Jong Eun Lee, Midori A Yenari
Journal of Cerebral Blood Flow & Metabolism 2007 May;28(1):53-63
Journal of Cerebral Blood Flow & Metabolism 2007 May;28(1):53-63
Differential regulation of Mn-superoxide dismutase in neurons and astroglia by HIV-1 gp120: Implications for HIV-associated dementia.
Saha RN, Pahan K
Free radical biology & medicine 2007 Jun 15;42(12):1866-78
Free radical biology & medicine 2007 Jun 15;42(12):1866-78
Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing in osteoclast precursors expression of LFA-1 and ICAM-1.
Garcia-Palacios V, Chung HY, Choi SJ, Sarmasik A, Kurihara N, Lee JW, Galson DL, Collins R, Roodman GD
Bone 2007 Feb;40(2):316-22
Bone 2007 Feb;40(2):316-22
NF-kappa B activation in human breast cancer specimens and its role in cell proliferation and apoptosis.
Biswas DK, Shi Q, Baily S, Strickland I, Ghosh S, Pardee AB, Iglehart JD
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 6;101(27):10137-42
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 6;101(27):10137-42
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
