GTX102090

antibody from GeneTex

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX102090

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102090, RRID:AB_10630493

Product name

NFkB p65 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.

Li HY, Leu YL, Wu YC, Wang SH
Journal of agricultural and food chemistry 2019 Feb 20;67(7):1889-1901

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NF-κB signaling and integrin-β1 inhibition attenuates osteosarcoma metastasis via increased cell apoptosis.

Li R, Shi Y, Zhao S, Shi T, Zhang G
International journal of biological macromolecules 2019 Feb 15;123:1035-1043

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SUR1-TRPM4 channel activation and phasic secretion of MMP-9 induced by tPA in brain endothelial cells.

Gerzanich V, Kwon MS, Woo SK, Ivanov A, Simard JM
PloS one 2018;13(4):e0195526

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Immunomodulatory Effects of the Nutraceutical Garlic Derivative Allicin in the Progression of Diabetic Nephropathy.

Arellano Buendía AS, Tostado González M, Sánchez Reyes O, García Arroyo FE, Argüello García R, Tapia E, Sánchez Lozada LG, Osorio Alonso H
International journal of molecular sciences 2018 Oct 11;19(10)

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Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.

Chen ST, Kuo TC, Liao YY, Lin MC, Tien YW, Huang MC
Oncogene 2018 Nov;37(46):6041-6053

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A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Endogenous H₂S production deficiencies lead to impaired renal erythropoietin production.

Leigh J, Juriasingani S, Akbari M, Shao P, Saha MN, Lobb I, Bachtler M, Fernandez B, Qian Z, Van Goor H, Pasch A, Feelisch M, Wang R, Sener A
Canadian Urological Association journal = Journal de l'Association des urologues du Canada 2018 Nov 20;

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Targeted γ-secretase inhibition of Notch signaling activation in acute renal injury.

Wyss JC, Kumar R, Mikulic J, Schneider M, Aebi JD, Juillerat-Jeanneret L, Golshayan D
American journal of physiology. Renal physiology 2018 May 1;314(5):F736-F746

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PCSK9 induces a pro-inflammatory response in macrophages.

Ricci C, Ruscica M, Camera M, Rossetti L, Macchi C, Colciago A, Zanotti I, Lupo MG, Adorni MP, Cicero AFG, Fogacci F, Corsini A, Ferri N
Scientific reports 2018 Feb 2;8(1):2267

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Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability.

Krga I, Tamaian R, Mercier S, Boby C, Monfoulet LE, Glibetic M, Morand C, Milenkovic D
Free radical biology & medicine 2018 Aug 20;124:364-379

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Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells.

Chamard-Jovenin C, Thiebaut C, Chesnel A, Bresso E, Morel C, Smail-Tabbone M, Devignes MD, Boukhobza T, Dumond H
Frontiers in endocrinology 2017;8:272

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Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model.

Liu MC, Chen WH, Chiou CS, Lo WC, Dubey NK, Chen YC, Lai WT, Yeh SD, Chiang HS, Deng WP
PloS one 2017;12(5):e0178152

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Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression.

Thiebaut C, Chamard-Jovenin C, Chesnel A, Morel C, Djermoune EH, Boukhobza T, Dumond H
PloS one 2017;12(3):e0173931

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Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis.

Gerzanich V, Makar TK, Guda PR, Kwon MS, Stokum JA, Woo SK, Ivanova S, Ivanov A, Mehta RI, Morris AB, Bryan J, Bever CT, Simard JM
Journal of neuroinflammation 2017 Sep 2;14(1):177

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Modulation of the Senescence-Associated Inflammatory Phenotype in Human Fibroblasts by Olive Phenols.

Menicacci B, Cipriani C, Margheri F, Mocali A, Giovannelli L
International journal of molecular sciences 2017 Oct 30;18(11)

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Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.

Wu PY, Lyu JL, Liu YJ, Chien TY, Hsu HC, Wen KC, Chiang HM
International journal of molecular sciences 2017 Oct 10;18(10)

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Immunomodulatory activities of curcumin-stabilized silver nanoparticles: Efficacy as an antiretroviral therapeutic.

Sharma RK, Cwiklinski K, Aalinkeel R, Reynolds JL, Sykes DE, Quaye E, Oh J, Mahajan SD, Schwartz SA
Immunological investigations 2017 Nov;46(8):833-846

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Immunomodulatory Role of Complement Proteins in the Neuropathology Associated with Opiate Abuse and HIV-1 Co-Morbidity.

Mahajan SD, Aalinkeel R, Parikh NU, Jacob A, Cwiklinski K, Sandhu P, Le K, Loftus AW, Schwartz SA, Quigg RJ, Alexander JJ
Immunological investigations 2017 Nov;46(8):816-832

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A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer.

Tsai CH, Tzeng SF, Hsieh SC, Yang YC, Hsiao YW, Tsai MH, Hsiao PW
Scientific reports 2017 Nov 15;7(1):15624

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VHL Inactivation in Precancerous Kidney Cells Induces an Inflammatory Response via ER Stress-Activated IRE1α Signaling.

Kuo CY, Lin CH, Hsu T
Cancer research 2017 Jul 1;77(13):3406-3416

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SP110b Controls Host Immunity and Susceptibility to Tuberculosis.

Leu JS, Chen ML, Chang SY, Yu SL, Lin CW, Wang H, Chen WC, Chang CH, Wang JY, Lee LN, Yu CJ, Kramnik I, Yan BS
American journal of respiratory and critical care medicine 2017 Feb 1;195(3):369-382

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Carbonic anhydrase IX inhibition affects viability of cancer cells adapted to extracellular acidosis.

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Journal of molecular medicine (Berlin, Germany) 2017 Dec;95(12):1341-1353

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Huang YC, Tsai MS, Hsieh PC, Shih JH, Wang TS, Wang YC, Lin TH, Wang SH
Toxicology and applied pharmacology 2017 Aug 15;329:128-139

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TrxR1 inhibition overcomes both hypoxia-induced and acquired bortezomib resistance in multiple myeloma through NF-кβ inhibition.

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Cell cycle (Georgetown, Tex.) 2016;15(4):559-72

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Functional Recovery in Osteoarthritic Chondrocytes Through Hyaluronic Acid and Platelet-Rich Plasma-Inhibited Infrapatellar Fat Pad Adipocytes.

Chen WH, Lin CM, Huang CF, Hsu WC, Lee CH, Ou KL, Dubey NK, Deng WP
The American journal of sports medicine 2016 Oct;44(10):2696-2705

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Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines.

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The Journal of experimental medicine 2015 Mar 9;212(3):333-49

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An enhancer element harboring variants associated with systemic lupus erythematosus engages the TNFAIP3 promoter to influence A20 expression.

Wang S, Wen F, Wiley GB, Kinter MT, Gaffney PM
PLoS genetics 2013;9(9):e1003750

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX102090 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, inculding GFP-tag) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX102090) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX102090) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NFkB p65 antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NFkB p65 antibody (GTX102090) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NFkB p65 antibody detects NFkB p65 protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NFkB p65 antibody (GTX102090) dilution: 1:500.