- GTX107678 - GeneTex RELA antibody

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ?g whole cell lysate)A:MOLT4 (GTX27912)7.5% SDS PAGEGTX107678 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g C8D30 whole cell lysate/extract C. 30 ?g NIH-3T3 whole cell lysate/extract 10% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract 7.5% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g K562 whole cell lysate/extract7.5% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000 and competitor's antibody (#4764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 £gg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 (GTX107678) antibody at 1:500 dilution.

GTX107678

Antibody data