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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA319553 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal NF-kB p65 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal NF-kB p65 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RELA
- Antibody clone number
- 27F9.G4
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
Submitted references HOX transcript antisense RNA is elevated in gastric carcinogenesis and regulated by the NF-κB pathway.
Zhang Z, Fan B, Liu F, Song N, Peng Y, Ma W, Ma R, Dong T, Liu S
Journal of cellular biochemistry 2019 Jun;120(6):10548-10555
Journal of cellular biochemistry 2019 Jun;120(6):10548-10555
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- anti NFKB p65 (Rel A) monoclonal antibody TA319553 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4?°C and detected with HRP conjugated Rb-anti Mouse antibody 1:40,000 in MB-070 30 min RT.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- monoclonal anti p65 TA319553 was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti mouse antibody at a dilution of 1:10000. Image provided courtesy of Dr. Al Baldwin, University of North Carolina, Chapel Hill.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by IF at a dilution of 1:5000. Hela cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Antibody TA319553 has been tested in IHC, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5 ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
- Validation comment
- IHC
