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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0546 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0546, RRID:AB_982740
- Product name
- RELA (phospho S536) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S536 of human RELA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Recruitment of NF-kappaB into mitochondria is involved in adenine nucleotide translocase 1 (ANT1)-induced apoptosis.
HTLV-I Tax induces a novel interaction between p65/RelA and p53 that results in inhibition of p53 transcriptional activity.
p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1.
Zamora M, Meroño C, Viñas O, Mampel T
The Journal of biological chemistry 2004 Sep 10;279(37):38415-23
The Journal of biological chemistry 2004 Sep 10;279(37):38415-23
HTLV-I Tax induces a novel interaction between p65/RelA and p53 that results in inhibition of p53 transcriptional activity.
Jeong SJ, Radonovich M, Brady JN, Pise-Masison CA
Blood 2004 Sep 1;104(5):1490-7
Blood 2004 Sep 1;104(5):1490-7
p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1.
Bohuslav J, Chen LF, Kwon H, Mu Y, Greene WC
The Journal of biological chemistry 2004 Jun 18;279(25):26115-25
The Journal of biological chemistry 2004 Jun 18;279(25):26115-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of RELA (phospho S536) polyclonal antibody (Cat # PAB0546) in mouse kidney tissue lysates (35 ug/lane). RELA (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with RELA (phospho S536) polyclonal antibody (Cat # PAB0546) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
