RQ4439

antibody from NSJ Bioreagents

Targeting: RELA NFKB3, p65

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4439

Provider

NSJ Bioreagents

Product name

p65 Antibody / NF-kB

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the p65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20deg;C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) COLO-320, 2) A549, 3) HepG2, 4) MDA-MB-231, 5) PANC-1 and 6) A375 lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat 1) spleen, 2) lung, 3) kidney, 4) testis and mouse 5) spleen, 6) lung, 7) testis and 8) NIH3T3 lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) COLO-320, 2) A549, 3) HepG2, 4) MDA-MB-231, 5) PANC-1 and 6) A375 lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat 1) spleen, 2) lung, 3) kidney, 4) testis and mouse 5) spleen, 6) lung, 7) testis and 8) NIH3T3 lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human Raji, 3) human HepG2, 4) human K562, 5) rat PC-12, 6) rat RH35, 7) mouse RAW264.7 and 8) mouse HEPA1-6 cell lysate with p65 antibody at 0.5ug/ml. Expected molecular weight ~65 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with p65 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon cancer with p65 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer with p65 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat small intestine with p65 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat kidney with p65 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with p65 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= p65 antibody.