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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- 200-301-065 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-065, RRID:AB_10893016
- Product name
- Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065
- Antibody type
- Monoclonal
- Vial size
- 100 µl
Submitted references Inter- and Intrapopulational Heterogeneity of Characteristic Markers in Adult Human Neural Crest-derived Stem Cells.
Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols.
A high-throughput pipeline for validation of antibodies.
Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice.
Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha.
Windmöller BA, Höving AL, Knabbe C, Greiner JFW
Stem cell reviews and reports 2022 Apr;18(4):1510-1520
Stem cell reviews and reports 2022 Apr;18(4):1510-1520
Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols.
Chansard A, Dubrulle N, Poujol de Molliens M, Falanga PB, Stephen T, Hasan M, van Zandbergen G, Aulner N, Shorte SL, David-Watine B
Frontiers in immunology 2020;11:569331
Frontiers in immunology 2020;11:569331
A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Suppression of Propionibacterium acnes Infection and the Associated Inflammatory Response by the Antimicrobial Peptide P5 in Mice.
Ryu S, Han HM, Song PI, Armstrong CA, Park Y
PloS one 2015;10(7):e0132619
PloS one 2015;10(7):e0132619
Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha.
Merkhofer EC, Cogswell P, Baldwin AS
Oncogene 2010 Feb 25;29(8):1238-48
Oncogene 2010 Feb 25;29(8):1238-48
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland monoclonal anti p65 200-301-065 was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti mouse antibody at a dilution of 1:10000. Image provided courtesy of Dr. Al Baldwin, University of North Carolina, Chapel Hill.
- Validation comment
- Western Blot
