HPA023887

antibody from Atlas Antibodies

Targeting: FYN MGC45350, SLK, SYN

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA023887

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023887, RRID:AB_1849226

Product name

Anti-FYN

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGT
LRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQ
ILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQ
AEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESET
TKGAYSLSIR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination.

Miyamoto Y, Torii T, Takada S, Ohno N, Saitoh Y, Nakamura K, Ito A, Ogata T, Terada N, Tanoue A, Yamauchi J
Molecular biology of the cell 2015 Oct 1;26(19):3489-503

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-FYN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells and lymphoid cells outside reaction centra.

Sample type

HUMAN