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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
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- Product number
- ARG62988 - Provider product page
- Provider
- Arigo
- Product name
- anti-Fyn antibody [FYN-01]
- Antibody type
- Monoclonal
- Antigen
- Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
- Description
- Purified from hybridoma culture supernatant by protein A-affinity chromatography.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- FYN-01
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at 2-8°C. Do not freeze. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Human primary fibroblasts stained with clone FYN-01 (green) Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with clone FYN-01 (green) Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: Murine transformed fibroblasts stained with clone FYN-01 (green) Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: T cells isolated from fresh buffy coats was immunoprecipitated with FYN-01 antibody. Lane 1: original lysate of T cells, Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent. Western blot was immunostained with anti-Fyn antibody (FYN-01).
