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FYN antibody from LifeSpan BioSciences, Inc.
MGC45350, SLK, SYN
- Western blot
Supportive data in Antibodypedia
- ELISA
Recommended by provider
- Immunocytochemistry
Data presented on provider website
- Immunohistochemistry
- Supportive data in Antibodypedia
Antibody data
- Antibody Data
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- Validations
- Western blot [2]
- Immunohistochemistry [16]
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- Product number
- LS-C800963
- Provider
- LifeSpan BioSciences, Inc.
- Product name
- SYN / FYN Antibody (phospho-Tyr530) LS-C800963
- Provider product page
- LifeSpan BioSciences, Inc. - LS-C800963
- Antibody type
- Polyclonal
- Description
- Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
No references: Submit reference
No comments: Submit comment
Supportive validation
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Src expression in various lysates
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Fyn phosphorylation expression in H2O2 treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
Supportive validation
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining human esophagus tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining human esophagus tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining human pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining human pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining rat spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1:200 staining rat spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
- Submitted by
- LifeSpan BioSciences, Inc. (provider)
- Main image
- Experimental details
- 1/100 staining human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
