NBP2-12919

antibody from Novus Biologicals

Targeting: TRPC5 PPP1R159

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-12919

Provider

Novus Biologicals

Product name

Mouse Monoclonal TRPC5 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 110kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TRPC5 Antibody (S67-15) [NBP2-12919] - Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody ) at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TRPC5 Antibody (S67-15) [NBP2-12919] - Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.