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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-45477 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC5 Monoclonal Antibody (N67/15), APC
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N67/15
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with TrpC5 monoclonal antibody (Product # MA5-45477) at 1:1,000 for Overnight at 4C, followed by Goat anti-mouse IgG:HRP at 1:7,000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpC5 monoclonal antibody (Product # MA5-45477) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 antibody (D) Composite.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Brain Slice. Fixation involved 10% formalin solution for 12-24 hours at RT. Samples were incubated with TrpC5 monoclonal antibody (Product # MA5-45477) at 1:1,000 for 1 hour at RT, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain used was Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.
