AF1707

antibody from R&D Systems

Targeting: CRP PTX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1707

Provider

R&D Systems

Product name

Human C-Reactive Protein/CRP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human C-Reactive Protein/CRP in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse CRP and 10% cross-reactivity with recombinant rat CRP is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P02741


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human C-Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue . PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for C-Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

C-Reactive Protein/CRP in BG01V Human Embryonic Stem Cells. C-Reactive Protein/CRP was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Sheep Anti-Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

C-Reactive Protein/CRP in Human Kidney Cancer Tissue. C-Reactive Protein/CRP was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 1.7 µg/mL Human C-Reactive Protein/CRP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1707) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.