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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2039 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2039, RRID:AB_1707299
- Product name
- PPP1R14A monoclonal antibody, clone 4H10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PPP1R14A.
- Isotype
- IgG
- Antibody clone number
- 4H10
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor.
Phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by protein kinase N.
Molecular cloning of a novel phosphorylation-dependent inhibitory protein of protein phosphatase-1 (CPI17) in smooth muscle: its specific localization in smooth muscle.
Lartey J, Smith M, Pawade J, Strachan B, Mellor H, López Bernal A
Biology of reproduction 2007 Jun;76(6):971-82
Biology of reproduction 2007 Jun;76(6):971-82
Phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by protein kinase N.
Hamaguchi T, Ito M, Feng J, Seko T, Koyama M, Machida H, Takase K, Amano M, Kaibuchi K, Hartshorne DJ, Nakano T
Biochemical and biophysical research communications 2000 Aug 11;274(3):825-30
Biochemical and biophysical research communications 2000 Aug 11;274(3):825-30
Molecular cloning of a novel phosphorylation-dependent inhibitory protein of protein phosphatase-1 (CPI17) in smooth muscle: its specific localization in smooth muscle.
Eto M, Senba S, Morita F, Yazawa M
FEBS letters 1997 Jun 30;410(2-3):356-60
FEBS letters 1997 Jun 30;410(2-3):356-60
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- The cell lysate of A-549 (30 ug) was resolved by SDS-PAGE and probed with PPP1R14A monoclonal antibody, clone 4H10 (Cat # MAB2039) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
