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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA303654 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303654, RRID:AB_2169540
- Product name
- Rabbit monoclonal antibody against AMPK alpha-1(Y365)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against AMPK alpha-1(Y365)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PRKAA1
- Isotype
- IgG
- Antibody clone number
- Y365
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - AMPK alpha 1 antibody [Y365]; All lanes : Anti-AMPK alpha 1 antibody [Y365] at 1/5000 dilution.Lane 1 : MCF-7.Lane 2 : Hela.Predicted band size : 63 kDa.Observed band size : 63 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - AMPK alpha 1 antibody [Y365]; TA303654 at a 1:100 - 1:250 dilution staining AMPK alpha 1 in Human cervical carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - Anti-AMPK alpha 1 antibody; Overlay histogram showing HeLa cells stained with TA303654 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
- Validation comment
- FC
