ARG54727

antibody from Arigo

Targeting: MTOR FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG54727

Provider

Arigo

Product name

anti-mTOR antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide around aa. 2459-2488 of Human mTOR protein (NP_004949.1).

Description

Protein A purified

Reactivity

Human

Host

Rabbit

Vial size

200 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 2 µg of 293 cell lysates 1) nontransfected or 2) transiently transfected with the FRAP1 gene stained with ARG54727 anti-mTOR antibody.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: Hela cell stained with ARG54727 anti-mTOR antibody followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: human testis tissue stained with ARG54727 anti-mTOR antibody.