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LearnPAB10335
antibody from Abnova Corporation
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10335 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10335, RRID:AB_1710183
- Product name
- MTOR (phospho S2448) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MTOR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin.
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.
Phospholipase D confers rapamycin resistance in human breast cancer cells.
Kristof AS, Marks-Konczalik J, Billings E, Moss J
The Journal of biological chemistry 2003 Sep 5;278(36):33637-44
The Journal of biological chemistry 2003 Sep 5;278(36):33637-44
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.
Nojima H, Tokunaga C, Eguchi S, Oshiro N, Hidayat S, Yoshino K, Hara K, Tanaka N, Avruch J, Yonezawa K
The Journal of biological chemistry 2003 May 2;278(18):15461-4
The Journal of biological chemistry 2003 May 2;278(18):15461-4
Phospholipase D confers rapamycin resistance in human breast cancer cells.
Chen Y, Zheng Y, Foster DA
Oncogene 2003 Jun 19;22(25):3937-42
Oncogene 2003 Jun 19;22(25):3937-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of MTOR (phospho S2448) polyclonal antibody (Cat # PAB10335) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human MTOR present in a 293T whole cell lysates.Cells were serum-starved for 24 hours prior to harvest.Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of Phospho-MTOR S2448 polyclonal antibody (Cat # PAB10335) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.This image shows moderate staining of proximal convoluted tubules of the kidney.Tissue was formalin-fixed and paraffin embedded.The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
