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LearnA00003-2
antibody from Boster Biological Technology
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A00003-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-mTOR/MTOR Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for mTOR/MTOR detection. Tested with WB, IHC-P, Direct ELISA in Human;Monkey.
- Reactivity
- Human, Simian
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references A Triple-Targeted Rutin-Based Self-Assembled Delivery Vector for Treating Ischemic Stroke by Vascular Normalization and Anti-Inflammation via ACE2/Ang1-7 Signaling.
The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma.
A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation.
The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.
Polyphyllin I Promotes Autophagic Cell Death and Apoptosis of Colon Cancer Cells via the ROS-Inhibited AKT/mTOR Pathway.
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts.
Zhao T, He F, Zhao K, Yuxia L, Li H, Liu X, Cen J, Duan S
ACS central science 2023 Jun 28;9(6):1180-1199
ACS central science 2023 Jun 28;9(6):1180-1199
The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma.
Sun Y, Yang X, Guan S, Ma T, Jiang Z, Gao M, Xu Y, Cong B
Archives of oral biology 2023 Dec;156:105810
Archives of oral biology 2023 Dec;156:105810
A Water-Soluble Quercetin Conjugate with Triple Targeting Exerts Neuron-Protective Effect on Cerebral Ischemia by Mitophagy Activation.
Cen J, Zhang R, Zhao T, Zhang X, Zhang C, Cui J, Zhao K, Duan S, Guo Y
Advanced healthcare materials 2022 Nov;11(22):e2200817
Advanced healthcare materials 2022 Nov;11(22):e2200817
The VEGFR2/mTOR/S6K1 pathway involved in the angiogenic effects of roxarsone in vitro and in vivo.
Zhang M, Ding L, Zhou Z, Liu C, Wang C, Chen B, Chen X, Zhang Y
Toxicology 2022 Aug;478:153290
Toxicology 2022 Aug;478:153290
Polyphyllin I Promotes Autophagic Cell Death and Apoptosis of Colon Cancer Cells via the ROS-Inhibited AKT/mTOR Pathway.
Luo Q, Jia L, Huang C, Qi Q, Jahangir A, Xia Y, Liu W, Shi R, Tang L, Chen Z
International journal of molecular sciences 2022 Aug 19;23(16)
International journal of molecular sciences 2022 Aug 19;23(16)
Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.
Zhang X, Zhang L, Chen Z, Li S, Che B, Wang N, Chen J, Xu C, Wei C
International journal of molecular medicine 2021 Mar;47(3)
International journal of molecular medicine 2021 Mar;47(3)
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.
Nie L, Liu M, Chen J, Wu Q, Li Y, Yi J, Zheng X, Zhang J, Chu C, Yang J
Molecular medicine reports 2021 Apr;23(4)
Molecular medicine reports 2021 Apr;23(4)
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
Kong L, Wu Y, Hu W, Liu L, Xue Y, Liang G
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro.
Wang P, Zhang SD, Jiao J, Wang W, Yu L, Zhao XL, Wang LT, Meng D, Fu YJ
Toxicology and applied pharmacology 2019 Sep 15;379:114647
Toxicology and applied pharmacology 2019 Sep 15;379:114647
Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts.
Zhai XX, Tang ZM, Ding JC, Lu XL
Molecular medicine reports 2017 Jun;15(6):3467-3472
Molecular medicine reports 2017 Jun;15(6):3467-3472
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of MTOR using anti-MTOR antibody (A00003-2).MTOR was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
