Explore
Validate
LearnALX-215-065-1
antibody from Enzo Life Sciences
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
Immunocytochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-215-065-1 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-215-065-1, RRID:AB_2051920
- Product name
- mTOR (human FRB Domain) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 vial
- Storage
- +4°C
Submitted references Organelle-specific, rapid induction of molecular activities and membrane tethering.
The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.
Komatsu T, Kukelyansky I, McCaffery JM, Ueno T, Varela LC, Inoue T
Nature methods 2010 Mar;7(3):206-8
Nature methods 2010 Mar;7(3):206-8
The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.
Haruki H, Nishikawa J, Laemmli UK
Molecular cell 2008 Sep 26;31(6):925-32
Molecular cell 2008 Sep 26;31(6):925-32
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot of mTOR (human FRB Domain), pAb (Prod. No. ALX-215-065) with Hela cells. Method:Two ml of an exponential HeLa suspension culture (2-4x10E5 cells) was washed twice with PBS quickly without resuspention of the pellet by spinning in a Eppendorf tube and finally with 25% TCA. TCA was carefully removed and pellet respuspended 100µl FSB + §ME (final sample buffer) and the pH adjusted with about a few µl of 1M Tris base (Å10 µl). The sample was heated at 100¡C for 5 min. and sonicated. SDS PAGE was at 7.5%, 20 & 25 µl loaded in lane 1 and 2, respectively. The TCA step is performed to prevent proteolytic breakdown of the very large mTOR.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- WB usingÊPAb to mTOR (human FRB Domain)Ê(Prod. No. ALX-215-065) on total yeast extracts derived from cells expressing FRB-tagged genes coding for three different transcription factors. Separation SDS-PAGE.
