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LearnNB100-240
antibody from Novus Biologicals
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-240 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-240, RRID:AB_10002801
- Product name
- Rabbit Polyclonal TOR/mTOR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Induction of autophagy and suppression of type I IFN secretion by CSFV.
Phospho-mTOR expression in human glioblastoma microglia-macrophage cells.
Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway.
Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients.
MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma.
High mTOR expression is associated with a worse oncological outcome in laryngeal carcinoma treated with postoperative radiotherapy: a pilot study.
Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation.
Xie B, Zhao M, Song D, Wu K, Yi L, Li W, Li X, Wang K, Chen J
Autophagy 2021 Apr;17(4):925-947
Autophagy 2021 Apr;17(4):925-947
Phospho-mTOR expression in human glioblastoma microglia-macrophage cells.
Lisi L, Ciotti GMP, Chiavari M, Pizzoferrato M, Mangiola A, Kalinin S, Feinstein DL, Navarra P
Neurochemistry international 2019 Oct;129:104485
Neurochemistry international 2019 Oct;129:104485
Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway.
Guo Y, Wang F, Li H, Liang H, Li Y, Gao Z, He X
Neurochemical research 2018 May;43(5):1111-1117
Neurochemical research 2018 May;43(5):1111-1117
Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients.
Suzuki H, Roa JC, Kawamoto T, Ishige K, Wistuba II, Li D, Thomas MB, Shoda J
Molecular and clinical oncology 2015 May;3(3):464-470
Molecular and clinical oncology 2015 May;3(3):464-470
MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma.
Liu DC, Yang ZL
Diagnostic cytopathology 2013 Mar;41(3):199-205
Diagnostic cytopathology 2013 Mar;41(3):199-205
High mTOR expression is associated with a worse oncological outcome in laryngeal carcinoma treated with postoperative radiotherapy: a pilot study.
Lionello M, Blandamura S, Loreggian L, Ottaviano G, Giacomelli L, Marchese-Ragona R, Velardita C, Staffieri A, Marioni G
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2012 Feb;41(2):136-40
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2012 Feb;41(2):136-40
Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation.
Bolster DR, Vary TC, Kimball SR, Jefferson LS
The Journal of nutrition 2004 Jul;134(7):1704-10
The Journal of nutrition 2004 Jul;134(7):1704-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 ug/ml (A) used for IP at 3 ug/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies NB100-241 and NB100-58821. For blotting immunoprecipitated mTOR, NB100-58821 was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
