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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX113385 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113385, RRID:AB_2036258
- Product name
- ARF1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113385 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 12% SDS PAGE GTX113385 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARF1 antibody detects ARF1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF1 antibody (GTX113385) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARF1 antibody detects ARF1 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF1 antibody (GTX113385) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ARF1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody (GTX113385) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ARF1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody (GTX113385) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using ARF1(GTX113385) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARF1 antibody detects ARF1 protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ARF1 antibody (GTX113385) diluted at 1:500.
