12745-1-AP

antibody from Invitrogen Antibodies

Targeting: MAP2K6 MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12745-1-AP

Provider

Invitrogen Antibodies

Product name

MEK6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSQSKGKKR NPGLKIPKEA FEQPQTSSTP PRDLDSKACI SIGNQNFEVK ADDLEPIMEL GRGAYGVVEK MRHVPSGQIM AVKRIRATVN SQEQKRLLMD LDISMRTVDC PFTVTFYGAL FREGDVWICM ELMDTSLDKF YKQVIDKGQT IPEDILGKIA VSIVKALEHL HSKLSVIHRD VKPSNVLINA LGQVKMCDFG ISGYLVDSVA KTIDAGCKPY MAPERINPEL NQKGYSVKSD IWSLGITMIE LAILRFPYDS WGTPFQQLKQ VVEEPSPQLP ADKFSAEFVD FTSQCLKKNS KERPTYPELM QHPFFTLHES KGTDVASFVK LILGD (1-334 aa encoded by BC012009)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12745-1-AP ( MEK6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12745-1-AP ( MEK6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug MEK6 antibody (12745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.