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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB1604 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat MKK6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat MKK6 in Western blots. In Western blots, no cross-reactivity with recombinant human MKK3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P52564.1- Isotype
- IgG
- Antibody clone number
- 304328
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat MKK6 by Western Blot. Western blot shows lysates of B16-F1 mouse melanoma cell line and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Human/Mouse/Rat MKK6 Monoclonal Antibody (Catalog # MAB1604) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. A specific band for MKK6 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
