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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- GTX84161 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84161, RRID:AB_10729674
- Product name
- SEK1 / MKK4 antibody [4B4]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-MAP2K4 mouse monoclonal antibody (GTX84161) immunofluorescent staining of COS7 cells transiently transfected with MAP2K4
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation (IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue using anti-MAP2K4 mouse monoclonal antibody. (GTX84161, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-MAP2K4mouse monoclonal antibody. (GTX84161, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-MAP2K4mouse monoclonal antibody. (GTX84161, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue using anti-MAP2K4mouse monoclonal antibody. (GTX84161, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either MAP2K4 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (GTX84161), and then analyzed by flow cytometry.
