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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX109675 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109675, RRID:AB_2037804
- Product name
- PUMA antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.
NF-κB-driven suppression of FOXO3a contributes to EGFR mutation-independent gefitinib resistance.
Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
FHIT loss confers cisplatin resistance in lung cancer via the AKT/NF-κB/Slug-mediated PUMA reduction.
Wei RJ, Lin SS, Wu WR, Chen LR, Li CF, Chen HD, Chou CT, Chen YC, Liang SS, Chien ST, Shiue YL
Toxicology and applied pharmacology 2016 Nov 15;311:88-98
Toxicology and applied pharmacology 2016 Nov 15;311:88-98
NF-κB-driven suppression of FOXO3a contributes to EGFR mutation-independent gefitinib resistance.
Chiu CF, Chang YW, Kuo KT, Shen YS, Liu CY, Yu YH, Cheng CC, Lee KY, Chen FC, Hsu MK, Kuo TC, Ma JT, Su JL
Proceedings of the National Academy of Sciences of the United States of America 2016 May 3;113(18):E2526-35
Proceedings of the National Academy of Sciences of the United States of America 2016 May 3;113(18):E2526-35
Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH
BMC cancer 2016 Jul 26;16:532
BMC cancer 2016 Jul 26;16:532
FHIT loss confers cisplatin resistance in lung cancer via the AKT/NF-κB/Slug-mediated PUMA reduction.
Wu DW, Lee MC, Hsu NY, Wu TC, Wu JY, Wang YC, Cheng YW, Chen CY, Lee H
Oncogene 2015 May 7;34(19):2505-15
Oncogene 2015 May 7;34(19):2505-15
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 0hr B: HCT116 cells with 30uM cisplatin treatment for 24hr 15% SDS PAGE GTX109675 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUMA antibody detects PUMA protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12 % SDS-PAGE, and blotted with PUMA antibody (GTX109675) diluted by 1:500
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PUMA antibody [C2C3], C-term immunoprecipitates BCL2 binding component 3 protein in IP experiments.IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 24 hrA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of BCL2 binding component 3 protein by 4 £gg PUMA antibody [C2C3], C-term (GTX109675)15 % SDS-PAGEThe immunoprecipitated BCL2 binding component 3 protein was detected by PUMA antibody [C2C3], C-term (GTX109675) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using PUMA(GTX109675) antibody at 1:500 dilution.
