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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP11313PU-N - Provider product page
- Provider
- OriGene
- Product name
- Bcl2 Binding component 3 (BBC3) (BH3 Domain) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- Bcl2 Binding component 3 (BBC3) (BH3 Domain) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- BBC3
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Puma Antibody (BH3 domain) in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Puma Antibody (BH3 domain) ?with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. RIGHT: Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma Antibody (BH3 domain) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of Hela cells using Puma Antibody (BH3 domain) ?(right histogram) compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
