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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- TA324837 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal IL1R Antibody (C-term E487)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal IL1R Antibody (C-term E487)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- IL1R1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.368 mg/ml
Submitted references GPER signalling in both cancer-associated fibroblasts and breast cancer cells mediates a feedforward IL1β/IL1R1 response.
De Marco P, Lappano R, De Francesco EM, Cirillo F, Pupo M, Avino S, Vivacqua A, Abonante S, Picard D, Maggiolini M
Scientific reports 2016 Apr 13;6:24354
Scientific reports 2016 Apr 13;6:24354
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-IL1R (Cat.#TA324837) in HL60 cell line lysates (35ug/lane). IL1R (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from HepG2 cell line, using IL1R-pY496(Cat. #TA324837). TA324837 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with IL1R antibody (C-term E487), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
