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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- BS-5394R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-IL-1R1 (Tyr496) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4°C. Followed by incubation with conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Mouse Intestinal lysate; Probed with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:300 overnight at 4°C. Followed by incubation with conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded Rat Colitis; Antigen retrieval performed with citrate buffer (0.01 M, pH 6.0), boiling for 15 min; Block endogenous peroxidase with 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5394R) at 1:200, Overnight at 4°C followed by conjugated secondary antibody, DAB and counterstaining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were fixed with 70% methanol (overnight at 4°C). The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Primary incubation with IL-1R1 (Tyr496) Polyclonal Antibody, Unconjugated (bs-5349R) at 1:100 (1 µg /10^6 cells) for 30 minutes at room temp. A PE-conjugated secondary antibody (bs-0295G-PE) was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (bs-0295P; orange).
