Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- 15421-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15421-1-AP, RRID:AB_2223741
- Product name
- ACLY antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ACLY antibody (Cat. #15421-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mitofusin 2 Deficiency Causes Pro-Inflammatory Effects in Human Primary Macrophages.
Novel SREBP1 inhibitor cinobufotalin suppresses proliferation of hepatocellular carcinoma by targeting lipogenesis.
Metformin alleviates inflammation through suppressing FASN-dependent palmitoylation of Akt.
Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation.
ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.
Nuclear-translocation of ACLY induced by obesity-related factors enhances pyrimidine metabolism through regulating histone acetylation in endometrial cancer.
Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration.
Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
ACLY facilitates colon cancer cell metastasis by CTNNB1.
Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
Adipocyte ACLY Facilitates Dietary Carbohydrate Handling to Maintain Metabolic Homeostasis in Females.
Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.
Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.
DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.
Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.
ACSS2-mediated acetyl-CoA synthesis from acetate is necessary for human cytomegalovirus infection.
The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Acetyl-coenzyme A carboxylase alpha promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.
Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma.
Mutations in CIC and IDH1 cooperatively regulate 2-hydroxyglutarate levels and cell clonogenicity.
Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1.
Khodzhaeva V, Schreiber Y, Geisslinger G, Brandes RP, Brüne B, Namgaladze D
Frontiers in immunology 2021;12:723683
Frontiers in immunology 2021;12:723683
Novel SREBP1 inhibitor cinobufotalin suppresses proliferation of hepatocellular carcinoma by targeting lipogenesis.
Meng H, Shen M, Li J, Zhang R, Li X, Zhao L, Huang G, Liu J
European journal of pharmacology 2021 Sep 5;906:174280
European journal of pharmacology 2021 Sep 5;906:174280
Metformin alleviates inflammation through suppressing FASN-dependent palmitoylation of Akt.
Xiong W, Sun KY, Zhu Y, Zhang X, Zhou YH, Zou X
Cell death & disease 2021 Oct 12;12(10):934
Cell death & disease 2021 Oct 12;12(10):934
Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation.
Trefely S, Huber K, Liu J, Noji M, Stransky S, Singh J, Doan MT, Lovell CD, von Krusenstiern E, Jiang H, Bostwick A, Pepper HL, Izzo L, Zhao S, Xu JP, Bedi KC Jr, Rame JE, Bogner-Strauss JG, Mesaros C, Sidoli S, Wellen KE, Snyder NW
Molecular cell 2021 Nov 22;
Molecular cell 2021 Nov 22;
ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.
Tian H, Shi S, You B, Zhang Q, Gu M, You Y
Journal of translational medicine 2021 Feb 16;19(1):77
Journal of translational medicine 2021 Feb 16;19(1):77
Nuclear-translocation of ACLY induced by obesity-related factors enhances pyrimidine metabolism through regulating histone acetylation in endometrial cancer.
Dai M, Yang B, Chen J, Liu F, Zhou Y, Zhou Y, Xu Q, Jiang S, Zhao S, Li X, Zhou X, Yang Q, Li J, Wang Y, Zhang Z, Teng Y
Cancer letters 2021 Aug 10;513:36-49
Cancer letters 2021 Aug 10;513:36-49
Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration.
Guilherme A, Yenilmez B, Bedard AH, Henriques F, Liu D, Lee A, Goldstein L, Kelly M, Nicoloro SM, Chen M, Weinstein L, Collins S, Czech MP
Cell reports 2020 May 5;31(5):107598
Cell reports 2020 May 5;31(5):107598
Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.
Zhao S, Jang C, Liu J, Uehara K, Gilbert M, Izzo L, Zeng X, Trefely S, Fernandez S, Carrer A, Miller KD, Schug ZT, Snyder NW, Gade TP, Titchenell PM, Rabinowitz JD, Wellen KE
Nature 2020 Mar;579(7800):586-591
Nature 2020 Mar;579(7800):586-591
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
He A, Chen X, Tan M, Chen Y, Lu D, Zhang X, Dean JM, Razani B, Lodhi IJ
Molecular cell 2020 Jul 2;79(1):30-42.e4
Molecular cell 2020 Jul 2;79(1):30-42.e4
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
Yang N, Li C, Li H, Liu M, Cai X, Cao F, Feng Y, Li M, Wang X
Frontiers in pharmacology 2019;10:709
Frontiers in pharmacology 2019;10:709
ACLY facilitates colon cancer cell metastasis by CTNNB1.
Wen J, Min X, Shen M, Hua Q, Han Y, Zhao L, Liu L, Huang G, Liu J, Zhao X
Journal of experimental & clinical cancer research : CR 2019 Sep 12;38(1):401
Journal of experimental & clinical cancer research : CR 2019 Sep 12;38(1):401
Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
Zhang Y, Li C, Hu C, Wu Q, Cai Y, Xing S, Lu H, Wang L, Huang, Sun L, Li T, He X, Zhong X, Wang J, Gao P, Smith ZJ, Jia W, Zhang H
EMBO reports 2019 Oct 4;20(10):e48115
EMBO reports 2019 Oct 4;20(10):e48115
Adipocyte ACLY Facilitates Dietary Carbohydrate Handling to Maintain Metabolic Homeostasis in Females.
Fernandez S, Viola JM, Torres A, Wallace M, Trefely S, Zhao S, Affronti HC, Gengatharan JM, Guertin DA, Snyder NW, Metallo CM, Wellen KE
Cell reports 2019 May 28;27(9):2772-2784.e6
Cell reports 2019 May 28;27(9):2772-2784.e6
Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.
Trefely S, Liu J, Huber K, Doan MT, Jiang H, Singh J, von Krusenstiern E, Bostwick A, Xu P, Bogner-Strauss JG, Wellen KE, Snyder NW
Molecular metabolism 2019 Dec;30:61-71
Molecular metabolism 2019 Dec;30:61-71
Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.
Namgaladze D, Zukunft S, Schnütgen F, Kurrle N, Fleming I, Fuhrmann D, Brüne B
Frontiers in immunology 2018;9:2858
Frontiers in immunology 2018;9:2858
DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.
Xu M, Wu H, Li M, Wen Y, Yu C, Xia L, Xia Q, Kong X
International journal of biological sciences 2018;14(13):1892-1900
International journal of biological sciences 2018;14(13):1892-1900
Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.
Liu X, Cooper DE, Cluntun AA, Warmoes MO, Zhao S, Reid MA, Liu J, Lund PJ, Lopes M, Garcia BA, Wellen KE, Kirsch DG, Locasale JW
Cell 2018 Oct 4;175(2):502-513.e13
Cell 2018 Oct 4;175(2):502-513.e13
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347
The EMBO journal 2017 May 15;36(10):1330-1347
Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Mews P, Donahue G, Drake AM, Luczak V, Abel T, Berger SL
Nature 2017 Jun 15;546(7658):381-386
Nature 2017 Jun 15;546(7658):381-386
Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.
Sivanand S, Rhoades S, Jiang Q, Lee JV, Benci J, Zhang J, Yuan S, Viney I, Zhao S, Carrer A, Bennett MJ, Minn AJ, Weljie AM, Greenberg RA, Wellen KE
Molecular cell 2017 Jul 20;67(2):252-265.e6
Molecular cell 2017 Jul 20;67(2):252-265.e6
ACSS2-mediated acetyl-CoA synthesis from acetate is necessary for human cytomegalovirus infection.
Vysochan A, Sengupta A, Weljie AM, Alwine JC, Yu Y
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):E1528-E1535
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):E1528-E1535
The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.
Li G, Li M, Hu J, Lei R, Xiong H, Ji H, Yin H, Wei Q, Hu G
Oncogene 2017 Feb 16;36(7):989-998
Oncogene 2017 Feb 16;36(7):989-998
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Gu Z, Zhang H, Li Y, Shen S, Yin X, Zhang W, Cheng R, Zhang Y, Zhang X, Chen H, Huang B, Cao Y
Oncotarget 2017 Dec 26;8(69):114328-114343
Oncotarget 2017 Dec 26;8(69):114328-114343
Acetyl-coenzyme A carboxylase alpha promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.
Wang MD, Wu H, Fu GB, Zhang HL, Zhou X, Tang L, Dong LW, Qin CJ, Huang S, Zhao LH, Zeng M, Wu MC, Yan HX, Wang HY
Hepatology (Baltimore, Md.) 2016 Apr;63(4):1272-86
Hepatology (Baltimore, Md.) 2016 Apr;63(4):1272-86
Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma.
Sun H, Zhu A, Zhang L, Zhang J, Zhong Z, Wang F
International journal of molecular sciences 2015 Oct 15;16(10):24574-87
International journal of molecular sciences 2015 Oct 15;16(10):24574-87
Mutations in CIC and IDH1 cooperatively regulate 2-hydroxyglutarate levels and cell clonogenicity.
Chittaranjan S, Chan S, Yang C, Yang KC, Chen V, Moradian A, Firme M, Song J, Go NE, Blough MD, Chan JA, Cairncross JG, Gorski SM, Morin GB, Yip S, Marra MA
Oncotarget 2014 Sep 15;5(17):7960-79
Oncotarget 2014 Sep 15;5(17):7960-79
Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1.
Yu Y, Maguire TG, Alwine JC
Journal of virology 2012 Mar;86(6):2942-9
Journal of virology 2012 Mar;86(6):2942-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 15421-1-AP(ACLY antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line