M02372

antibody from Boster Biological Technology

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02372

Provider

Boster Biological Technology

Product name

Anti-ATP citrate lyase ACLY Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for ATP CITRATE LYASE/ACLY detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ATP CITRATE LYASE/ACLY information: Molecular Weight: 120839 MW; Subcellular Localization: Cytoplasm.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

ECF-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

FGF21 Reduces Lipid Accumulation in Bovine Hepatocytes by Enhancing Lipid Oxidation and Reducing Lipogenesis via AMPK Signaling.

Kong Y, Zhao C, Tan P, Liu S, Huang Y, Zeng F, Ma P, Guo Y, Zhao B, Wang J
Animals : an open access journal from MDPI 2022 Apr 6;12(7)

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Elucidation of the mechanism of NEFA-induced PERK-eIF2α signaling pathway regulation of lipid metabolism in bovine hepatocytes.

Huang Y, Zhao C, Kong Y, Tan P, Liu S, Liu Y, Zeng F, Yuan Y, Zhao B, Wang J
The Journal of steroid biochemistry and molecular biology 2021 Jul;211:105893

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody (M02372)ACLY was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACLY Antibody (M02372)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image