15421-1-AP

antibody from Invitrogen Antibodies

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15421-1-AP

Provider

Invitrogen Antibodies

Product name

ATP citrate lyase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MFSSEVQFG HAGACANQAS ETAVAKNQAL KEAGVFVPRS FDELGEIIQS VYEDLVANGV IVPAQEVPPP TVPMDYSWAR ELGLIRKPAS FMTSICDERG QELIYAGMPI TEVFKEEMGI GGVLGLLWFQ KRLPKYSCQF IEMCLMVTAD HGPAVSGAHN TIICARAGKD LVSSLTSGLL TIGDRFGGAL DAAAKMFSKA FDSGIIPMEF VNKMKKEGKL IMGIGHRVKS INNPDMRVQI LKDYVRQHFP ATPLLDYALE VEKITTSKKP NLILNVDGLI GVAFVDMLRN CGSFTREEAD EYIDIGALNG IFVLGRSMGF IGHYLDQKRL KQGLYRHPWD DISYVLPEHM SM (751-1101 aa encoded by BC006195)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug ATP citrate lyase antibody (15421-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ATP citrate lyase (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:1000) with K-562 cells lysate 3200ug.