835803

antibody from BioLegend

Targeting: NEFH NF-H, NFH

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

835803

Provider

BioLegend

Proper citation

BioLegend Cat#835803, RRID:AB_2715862

Product name

Purified anti-Neurofilament H (NF-H)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

SMI 37

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

IHC staining of anti-Neurofilament H (NF-H) antibody (clone SMI 37) on formalin-fixed paraffin-embedded rat brain tissue. After antigen retrieval using Retrieve-All Antigen Unmasking System 3, the tissue was incubated with the primary antibody at 5 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection, followed by hematoxylin counterstaining.

Conjugate

Unconjugated