PA3-16753

antibody from Invitrogen Antibodies

Targeting: NEFH NF-H, NFH

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-16753

Provider

Invitrogen Antibodies

Product name

NF-H Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat, Bovine, Canine, Feline, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

50 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

SLC13A5/sodium-citrate co-transporter overexpression causes disrupted white matter integrity and an autistic-like phenotype.

Rigby MJ, Orefice NS, Lawton AJ, Ma M, Shapiro SL, Yi SY, Dieterich IA, Frelka A, Miles HN, Pearce RA, Yu JPJ, Li L, Denu JM, Puglielli L
Brain communications 2022 Feb;4(1):fcac002

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Degeneration and regeneration of corneal nerves in response to HSV-1 infection.

Chucair-Elliott AJ, Zheng M, Carr DJ
Investigative ophthalmology & visual science 2015 Jan 13;56(2):1097-107

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-H in spinal cord lysates from different species. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753 using a dilution of 1:20,000. Antibody in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220 kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NF-H in 200 kDa Neurofilament Heavy expression in rat spinal cord extract. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753). Lane 1: Coomassie Brilliant Blue stained; Lane 2: probed with chicken anti-Neurofilament Heavy antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of NF-H in mixed rat neuron/glial cultures. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753). Neurofilament Light (NF-L) antibody [green] and Neurofilament Heavy (NF-H) antibody. Blue is a DNA stain. NF-H antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of NF-H in SH-SY5Y cells. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753). Neurofilament Heavy Antibody (red) and Fibrillarin Antibody (green). Nuclear DNA is stained with Hoechst dye (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of NF-H in Rat neurons. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NF-H in rat cerebellum sections. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753) using a dilution of 1:5,000 (Red). Costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hrs, cut to 45 µM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NF-H in rat cerebellum sections. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16753) using a dilution of 1:5,000 (Red). Costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hrs, cut to 45 µM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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