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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-29777 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pirin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse liver. Predicted reactivity: Mouse (95%), Rat (96%), Rhesus Monkey (98%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Pirin Polyclonal Antibody (Product # PA5-29777). Sample (30 µg of whole cell lysate). Lane A: Hela. 12% SDS PAGE. Pirin Polyclonal Antibody . Pirin Polyclonal Antibody (Product # PA5-29777) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pirin Polyclonal Antibody detects PIR protein by Western blot analysis. A. 50 µg mouse liver lysate/extract.12 % SDS-PAGE. Pirin Polyclonal Antibody (Product # PA5-29777) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Pirin in paraformaldehyde-fixed HeLa cells using a Pirin polyclonal antibody (Product # PA5-29777) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MPG xenograft, using Pirin (Product # PA5-29777) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pirin Polyclonal Antibody detects Pirin protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Pirin Polyclonal Antibody (Product # PA5-29777) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pirin Polyclonal Antibody detects Pirin protein at cytosol and nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. Pirin Polyclonal Antibody (Product # PA5-29777) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
