Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-27138 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCGR2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: THP-1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Increased FcγRIIB dominance contributes to the emergence of resistance to therapeutic antibodies in chronic lymphocytic leukaemia patients.
Burgess M, Mapp S, Mazzieri R, Cheung C, Chambers L, Mattarollo SR, Mollee P, Gill D, Saunders NA
Oncogene 2017 Apr 27;36(17):2366-2376
Oncogene 2017 Apr 27;36(17):2366-2376
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FCGR2B Polyclonal Antibody (Product # PA5-27138). Sample (30 µg of whole cell lysate). Lane A: THP-1. 10% SDS PAGE. FCGR2B Polyclonal Antibody (Product # PA5-27138) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD32B/FCGR2B in methanol-fixed A431 cells using a CD32B/FCGR2B polyclonal antibody (Product # PA5-27138) at a 1:500 dilution.