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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF1330 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fc gamma RII/CD32 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Fc gamma RIIB and rhFc gamma RIIIA is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P31994- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Antibody-Dependent Enhancement of Ebola Virus Infection by Human Antibodies Isolated from Survivors.
Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.
Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia.
C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression.
Compromised NK cell-mediated antibody-dependent cellular cytotoxicity in chronic SIV/SHIV infection.
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.
Self double-stranded (ds)DNA induces IL-1β production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies.
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
Kuzmina NA, Younan P, Gilchuk P, Santos RI, Flyak AI, Ilinykh PA, Huang K, Lubaki NM, Ramanathan P, Crowe JE Jr, Bukreyev A
Cell reports 2018 Aug 14;24(7):1802-1815.e5
Cell reports 2018 Aug 14;24(7):1802-1815.e5
Multivalent Fcγ-receptor engagement by a hexameric Fc-fusion protein triggers Fcγ-receptor internalisation and modulation of Fcγ-receptor functions.
Qureshi OS, Rowley TF, Junker F, Peters SJ, Crilly S, Compson J, Eddleston A, Björkelund H, Greenslade K, Parkinson M, Davies NL, Griffin R, Pither TL, Cain K, Christodoulou L, Staelens L, Ward E, Tibbitts J, Kiessling A, Smith B, Brennan FR, Malmqvist M, Fallah-Arani F, Humphreys DP
Scientific reports 2017 Dec 6;7(1):17049
Scientific reports 2017 Dec 6;7(1):17049
Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia.
McWilliams EM, Mele JM, Cheney C, Timmerman EA, Fiazuddin F, Strattan EJ, Mo X, Byrd JC, Muthusamy N, Awan FT
Oncoimmunology 2016;5(10):e1226720
Oncoimmunology 2016;5(10):e1226720
C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression.
Kim Y, Ryu J, Ryu MS, Lim S, Han KO, Lim IK, Han KH
FEBS letters 2014 Feb 14;588(4):625-31
FEBS letters 2014 Feb 14;588(4):625-31
Compromised NK cell-mediated antibody-dependent cellular cytotoxicity in chronic SIV/SHIV infection.
He X, Li D, Luo Z, Liang H, Peng H, Zhao Y, Wang N, Liu D, Qin C, Wei Q, Yan H, Shao Y
PloS one 2013;8(2):e56309
PloS one 2013;8(2):e56309
Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.
Emmerich P, Mika A, Schmitz H
PLoS neglected tropical diseases 2013;7(12):e2580
PLoS neglected tropical diseases 2013;7(12):e2580
Self double-stranded (ds)DNA induces IL-1β production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies.
Shin MS, Kang Y, Lee N, Wahl ER, Kim SH, Kang KS, Lazova R, Kang I
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1407-15
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1407-15
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia 2011 Oct;54(10):2713-23
Diabetologia 2011 Oct;54(10):2713-23
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
Journal of leukocyte biology 2008 Oct;84(4):1109-19
Journal of leukocyte biology 2008 Oct;84(4):1109-19
C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
Atherosclerosis 2008 Jan;196(1):129-35
Atherosclerosis 2008 Jan;196(1):129-35
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fc gamma RII/CD32 in Human PBMCs. Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
