Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-487 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-487, RRID:AB_2121991
- Product name
- Rabbit Polyclonal IGBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The E3 ubiquitin ligase midline 1 promotes allergen and rhinovirus-induced asthma by inhibiting protein phosphatase 2A activity.
The E3 ubiquitin ligase- and protein phosphatase 2A (PP2A)-binding domains of the Alpha4 protein are both required for Alpha4 to inhibit PP2A degradation.
Collison A, Hatchwell L, Verrills N, Wark PA, de Siqueira AP, Tooze M, Carpenter H, Don AS, Morris JC, Zimmermann N, Bartlett NW, Rothenberg ME, Johnston SL, Foster PS, Mattes J
Nature medicine 2013 Feb;19(2):232-7
Nature medicine 2013 Feb;19(2):232-7
The E3 ubiquitin ligase- and protein phosphatase 2A (PP2A)-binding domains of the Alpha4 protein are both required for Alpha4 to inhibit PP2A degradation.
LeNoue-Newton M, Watkins GR, Zou P, Germane KL, McCorvey LR, Wadzinski BE, Spiller BW
The Journal of biological chemistry 2011 May 20;286(20):17665-71
The Journal of biological chemistry 2011 May 20;286(20):17665-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: IGBP1 Antibody [NB100-487] - Detection of Alpha-4 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg) from Ramos (R), K562 (K) and mouse NIH3T3 (M) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP reaction loaded/lane) from Ramos cells. Antibodies: NB 100-487 used at 0.4 mcg/ml (A) and 1 mcg/ml (B) for WB at 3 mcg/mg lysate for IP. Alpha-4 was also immunoprecipitated by NB 100-486, but not by another Alpha-4 antibody, which are both affinity purified antibodies to upstream epitopes on Alpha-4. Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).