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- Antigen structure
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- Validations
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- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-24171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Serum Amyloid P Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines.
Behrens NE, Lipke PN, Pilling D, Gomer RH, Klotz SA
mBio 2019 Mar 12;10(2)
mBio 2019 Mar 12;10(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an APCS polyclonal antibody (Product # PA5-24171) in K562 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human hepatocarcinoma using an APCS polyclonal antibody (Product # PA5-24171), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG 1 Binding and phagocytosis of yeasts by human macrophages. (A) SAP binding to C. albicans yeast cells determined by SAP antibody labeling and flow cytometry. (B) SAP binding to yeast cells of C. albicans , S. cerevisiae -EV, the amyloidogenic S. cerevisiae - Als5p WT , and the nonamyloidogenic, S. cerevisiae - Als5p V326N . SAP was offered to yeasts in normal human serum. Each symbol is the average of one of seven independent experiments. (C, left) Binding of fungi to macrophages upon the addition of C. albicans , S. cerevisiae - EV, S. cerevisiae - Als5p WT , or S. cerevisiae - Als5p V326N . (Right) Phagocytosis of the same strains of yeast cells by macrophages. Each symbol is the average from three independent experiments. (D) Effect of SAP on macrophage binding and phagocytosis of C. albicans . (Top left) Binding of yeast cells to control macrophages or macrophages pretreated with SAP. (Top right) Phagocytosis of C. albicans by control macrophages or macrophages pretreated with SAP (30 µg/mL). (Bottom left) Binding of yeast cells in serum-free medium and yeast cells offered SAP in normal human serum to macrophages. (Bottom right) Phagocytosis by macrophages of yeast cells in serum-free medium and yeast cells offered SAP in normal human serum (results of three separate experiments each with 3,200 macrophages counted/variable). Note that the y axis is 10-fold higher for phagocytosis than the binding graphs. Statistical significance was determined by Mann-Whitney U
