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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10798 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MAP3K5 monoclonal antibody, clone 2E4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant MAP3K5.
- Isotype
- IgG
- Antibody clone number
- 2E4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) against human MAP3K5 (aa: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and prostate tissues (B) using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using MAP3K5 monoclonal antibody, clone 2E4 (Cat # MAB10798) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
